1095 The Utility of Plasma Circulating Cell-Free Messenger RNA as A Biomarker of Glioma: A Pilot Study

Abstract Introduction Research into the potential utility of plasma-derived circulating-cell-free nucleic acids as non-invasive adjuncts to radiological imaging has been occasioned by invasive nature brain tumour biopsy. Circulating-cell-free messenger RNAs are short fragments RNA present in blood. The objective this study was determine whether significant differences exist plasma transcriptomic profile glioma patients relative their characteristics, and also any observed were representative synchronously obtained samples TCGA derived RNA. Method Blood collected from twenty-nine prior resection. Plasma-ccfmRNA extracted profiled. Results BCL2L1, CXCL5, GZMB, HLA-A, HLA-C, IRF1, MYD88, TGFB1, TLR2, TP53 genes significantly over-expressed (high-grade-glioma-HGG low-grade-glioma-LGG) (p < 0.05, versus control). GZMB HLA-A HGG LGG patients). There positive correlation between magnitude fold change differentially expressed (Spearman r = 0.6344, n 14, p 0.017), with mean FPKM 0.4614, 19, 0.047). radiographic burden CSF3 gene (r 0.9813, 20, 0.001). Conclusions We identified differential expression involved cancer inflammation immunity among different grades, we samples,